Table 1.
Effect of various substances on sequestration of surface-bound testosterone-BSA-FITC
| Substance | Fluorescence intensity (%) | Surface-bound fluorescence | Internalized fluorescence |
|---|---|---|---|
| Control | 100 | + | + |
| NaN3 (0.04%, 30 min) | 62.9 ± 6.5 | + | − |
| Pertussis toxin (500 ng/ml, 16 h) | 70.4 ± 0.5 | + | − |
| U-73122 (2 μM, 2 min) | 89.2 ± 5.8 | + | − |
| U-73343 (2 μM, 2 min) | 101.8 ± 5.4 | + | + |
| Nocodazole (2.5 μg/ml, 15 min) | 72.0 ± 5.5 | + | − |
| Cytochalasin B (10 μg/ml, 15 min) | 72.2 ± 5.4 | + | − |
Intact IC-21 cells (1.5 × 106) were incubated with the different substances at the indicated concentrations for the indicated periods before testosterone-BSA-FITC was added for 15 min. Thereafter, cells were fixed, and fluorescence intensity was evaluated by flow cytometry and fluorescence localization by CLSM. Values normalized to controls are given as means ± SD from at least two different experiments.