Skip to main content
. 2008 Jun 10;112(7):2858–2868. doi: 10.1182/blood-2008-03-145946

Figure 5.

Figure 5

Depletion of Treg cells by HIV-1 was associated with induction of apoptosis. Cells from spleen (A) and mLN (B) in HIV-1– or mock-infected mice were harvested; human FoxP3+ Treg cells and FoxP3− T cells (CD45+CD3+CD8−) were analyzed for the expression of activated caspase-3 in the spleen and mLN. Numbers indicate percentage active caspase-3+ cells. Numbers in parentheses indicate relative caspase-3 signals of total CD3+CD8−FoxP3+ or CD3+CD8−FoxP3− T cells. Summarized data of percentage of active caspase-3 of FoxP3+ Treg cells or FoxP3− T cells in spleen (C) and mLN (D) are shown. (E-G) Induction of apoptosis in HIV-infected (p24+) and bystander (p24−) cells of FoxP3+ or FoxP3− (CD3+CD8−) T cells. (E) FACS plots with mLN CD45+CD3+CD8− T cells from mock- and HIV-infected DKO-hu mice. Numbers indicate percentage of cells in each subpopulation. Relative induction of apoptosis in p24+ and p24− FoxP3+ Treg cells (F) and FoxP3− T cells (G) is summarized. Data shown are representative of 3 independent experiments with at least 3 mice in each group. Error bars indicate standard errors. *P < .05.