Figure 12.
Comparison of quadruple (a) and triple (b) exponential fitting of the kinetics of refolding of F45W ubiquitin at final GuHCl concentrations of 0.5 and 1.0 M (pH 5, 25 °C). Fluorescence traces measured in continuous- and stopped-flow experiments were normalized with respect to the unfolded protein in 6 M GuHCl. The residuals (top two traces in each panel) indicate that four exponentials are required to obtain a satisfactory fit of the data over the time window shown. Reprinted with permission from ref 14. Copyright 2005 Wiley-VCH.