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. Author manuscript; available in PMC: 2008 Sep 30.
Published in final edited form as: Chem Rev. 2006 May;106(5):1836–1861. doi: 10.1021/cr040430y

Figure 8.

Figure 8

Stopped-flow fluorescence evidence for an unresolved rapid process (burst phase) during folding of cyt c (pH 5, 10 °C). a Tryptophan fluorescence changes during refolding of acid-unfolded cytochrome c (pH 2, ~15 mM HCl) at a final GuHCl concentration of 0.7 M. The initial signal S(0) at t=0 (determined on the basis of a separate dead-time measurement) falls short of the signal for the unfolded state under refolding conditions, Spred(U), obtained by linear extrapolation of the unfolded-state baseline (see dashed line in b). b Effect of the denaturant concentration on the initial (squares) and final (circles) fluorescence signal, S(0) and S(∞), measured in a series of stopped-flow refolding experiments at different final GuHCl concentration. Reprinted with permission from ref 138. Copyright 2004 Elsevier Inc.