Table 1.
Strains used in this study
| Strain | Relevant genotype | Source |
|---|---|---|
| Diploid | ||
| YPK4.7 | MATa/MATα ura3-52/ura3-52 lys2-801amber/lys2-801amber ade2-101ochre/ade2-101ochre trp1-Δ63/trp1-Δ63 his3-Δ200/his3-Δ200 leu2-Δ1/leu2-Δ1 | Kirchman et al., 1999 |
| YSK631 | YPK4.7 with +/rpd3Δ::URA3 +/hda1Δ::HIS3 | This study |
| YSK668 | YPK4.7 with +/rpd3Δ::URA3 +/sir3Δ::LEU2 | This study |
| Haploid | ||
| YSK661 | MATα rpd3Δ::URA3;haploid segregant of YSK631 | This study |
| YSK662 | MATa rpd3Δ::URA3 hda1Δ::HIS3; haploid segregant of YSK631 | This study |
| YSK663 | MATα; haploid segregant of YSK631 | This study |
| YSK664 | MATα hda1Δ::HIS3; haploid segregant of YSK631 | This study |
| YSK710 | MATα rpd3Δ::URA3 sir3Δ::LEU2; haploid segregant of YSK668 | This study |
| YSK711 | MATa sir3Δ::LEU2; haploid segregant of YSK668 | This study |
| YSK712 | MATa; haploid segregant of YSK668 | This study |
| YSK713 | MATα rpd3Δ::URA3; haploid segregant of YSK668 | This study |
| YSK770 | YSK663 with sir3Δ::LEU2 | This study |
| YSK771 | YSK664 with sir3Δ::LEU2 | This study |
| DY2126 | MATα ade2 can1 his3 leu2 trp1 ura3 hmr::TRP1 | Jiang and Stillman, 1996 |
| YSK694 | DY2126 with hda1Δ::HIS3 | This study |
| YSK696 | DY2126 with rpd3Δ::URA3 | This study |
| YSK726 | DY2126 with sir3Δ::LEU2 | This study |
| YSK730 | DY2126 with hda1Δ::HIS3 sir3Δ::LEU2 | This study |
| YSK728 | DY2126 with rpd3Δ::URA3 sir3Δ::LEU2 | This study |
| YDS21U | MATa ade2-1 can1-100 his3-11, 15 leu2-3, 112 trp1-1 URA3::TelVR (2.1 kb from Tel) | Rundlett et al., 1996 |
| YSK831 | YDS21U with hda1Δ::HIS3 | This study |
| YSK830 | YDS21U with rpd3Δ::TRP1 | This study |
| YSK829 | YDS21U with sir3Δ::LEU2 | This study |
| YSK833 | YDS21U with hda1Δ::HIS3 sir3Δ::LEU2 | This study |
| YSK832 | YDS21U with rpd3Δ::TRP1 sir3Δ::LEU2 | This study |
| M1 | MATα his3-Δ200 leu2-Δ1 ura3-167 trp1-Δ63 met15-Δ1 RDN1::Ty1-MET15 | Smith and Boeke, 1997 |
| M9 | Same as M1 except that Ty1-MET15 is inserted in a non-rDNA locus | Smith and Boeke, 1997 |
| JS218 | M1 with sir2::HIS3 | Smith and Boeke, 1997 |
| YSK757 | M1 with sir3Δ::LEU2 | This study |
| YSK753 | M1 with hda1Δ::pRS405 (LEU2) | This study |
| YSK781 | M1 with sir3Δ::LEU2 hda1Δ::pRS403 (HIS3) | This study |
| YSK755 | M1 with rpd3Δ::pRS404 (TRP1) | This study |
| YSK779 | JS218 with rpd3Δ::pRS306 (URA3) | This study |
| YCYL11 | M9 with rpd3Δ::pRS404 (TRP1) | This study |
| YSK783 | M1 with sir3Δ::LEU2 rpd3Δ::pRS406 (URA3) | This study |
| YPK9 | MATa ura3-52 lys2-801amber ade2-101ochre trp1-Δ63 his3-Δ200 leu2-Δ1 | From YPK4.7 (Kirchman et al., 1999) |
| YAB11 | YPK9 with sir2::URA3 | This study |
| YAB12 | YPK9 with rpd3Δ::pRS306 (URA3) | This study |
| YAB13 | YAB11 with rpd3Δ::pRS403 (HIS3) | This study |
| X2180-1A | MATa SUC2 mal1 mel1 gal2 CUP1 | The Berkeley Yeast Genetic Stock Center |