Table 2.
MSP2 Locus | Lane loading volume | ||||||
13 ul (B) | 20 ul | 30 ul | |||||
3D7/IC | 13 μl (A) | + | - | + | - | + | - |
+ | 79 | 1 | 74 | 6 | 73 | 7 | |
- | 1 | 12 | 1 | 1 | 0 | 2 | |
* P | 1.000 | 0.125 | 0.016 | ||||
FC27 | 13 μl (A) | + | - | + | - | + | - |
+ | 84 | 2 | 71 | 14 | 65 | 21 | |
- | 1 | 1 | 1 | 0 | 1 | 0 | |
* P | 1.000 | 0.001 | < 0.001 |
* McNemar's test run for 13 μl lane (A) compared to other lanes. For both 3D7/IC and FC27, the number of alleles detected was greatest when 13 μl loading volume was used