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. 2008 Sep 29;205(10):2199–2206. doi: 10.1084/jem.20080579

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© 2008 de Yébenes et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jem.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 3. — AID is a direct target of miR-181b. (A) Luciferase reporter assays of NIH-293T cells transfected with luciferase constructs containing AID–miR-181b putative binding sites. Cells were cotransfected with miR-181b miRNA duplexes along with wild-type (BS1 and BS2) or mutated versions (mutBS1 and mutBS2) of the miR-181b putative binding sites in the mouse AID 3′UTR. The sequences included in each luciferase construct are shown in Table S3 (available at http://www.jem.org/cgi/content/full/jem.20080579/DC1). Bars represent luciferase activity for each of the binding sites relative to their mutated counterparts (for raw data see Fig. S9). Statistical bars show SDs. P-values (paired two-tailed t test) are shown. (B) miR-181b and AID mRNA expression assessed by quantitative RT-PCR in Burkitt (closed triangles), diffuse large B cell lymphoma (closed circles), and Mantle cell lymphoma (open diamonds) human B cell lines. The expression of miR-181b and AID was normalized to their expression in primary human B cells, which has been given an arbitrary value of 1.