Table I.
Antibodies used in this study
| Antibody | Method | Concentration | Source | Isotype | Source |
|---|---|---|---|---|---|
| WNV-NS-1-biotin+ | IHC | 1:100 | R. Hall (University of Queensland, Brisbane, Australia) |
Mouse IgG1 | BioLegend |
| GS-lectin-biotin+/FITC | IHC | 1:50 | Sigma-Aldrich | N/A | N/A |
| CD11b-APC/PE/APC-CY7/Alexa-700 | FC | 1:100 | BD Biosciences/ BioLegend |
Rat IgG2b | BD Biosciences/ BioLegend |
| CD45-PerCP | FC | 1:100 | BD Biosciences | Rat IgG2b | BD Biosciences |
| Ly6C-FITC/ Biotin | FC | 1:100 | BD Biosciences | Rat IgM | BD Biosciences |
| Ly6G-PE | FC | 1:100 | BD Biosciences | BD Biosciences | |
| GR1-biotin/APC-Cy7/ Pacific blue | FC/IHC | 1:100 | BD Biosciences/ BioLegend |
Rat IgG2b | BD Biosciences/ BioLegend |
| CD11c-biotin+/PE-Cy7 | FC | 1:150 | BD Biosciences/ Biolegend |
Hamster IgG | BD Biosciences/ BioLegend |
| CCL2-LEAF | Neut | 100 μg per mouse | BioLegend | Hamster IgG-LEAF | BioLegend |
| CD115-PE | FC | 1:50 | eBioscience | Rat IgG2A | BioLegend |
FC, flow cytometry; Neut, neutralization; biotin+, biotinylated.