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The RPN11/MPR1 gene complements the thermosensitive phenotype of the mpr1-1 mutant strain. The plasmid YCpMPR1 and the control empty centromeric plasmid pYC (see MATERIALS AND METHODS) were transferred into the wild-type W303-MPR1 and mutant (W303-mpr1) strains. One transformant from each experiment was streaked on YPD and YPG and incubated at 24 and 36°C for 3 d.
