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. 1998 Oct;9(10):2917–2931. doi: 10.1091/mbc.9.10.2917

Figure 2.

Figure 2

RPN11/MPR1 sequence (GenBank accession number X79561), its deduced amino acid sequence, and sequence of the mpr1-1 allele. The HpaI site (double underlined) was used to insert the URA3 gene to interrupt the RPN11/MPR1 gene. The oligonucleotides IX13 and IX14 (bold) were used to amplify the mutant allele mpr1-1, which contained a CC to GCA change at nucleotides 1388–1389 (the Sau3A site is underlined); the mutated sequence is indicated above the line. The oligonucleotides 5′EcoRIMPR and 3′EcoRIMPR (underlined) were used to amplify the wild-type allele to fuse RPN11/MPR1 to GFP (see MATERIALS AND METHODS). The amino acid sequence that forms a putative coiled-coils domain is shown in bold type.