Table 1.

Yeast strains and plasmids

Strain	Genotype	Source
W303	MATaMATα, his3-11/his3-11, ade2-1/ade2-1, leu2-3,112/leu2-3,112, ura3-1/ura3-1,trp1-Δ2/trp1-Δ2, can1-100 [rho+]	Thomas and Rothstein, 1989
W303-1B	MATα, his3-11, ade2-1, leu2-3,112, ura3-1, trp1-Δ2, can1-100 [rho+]	Thomas and Rothstein, 1989
R117	MATa/MATα, his1, ura1,2 MPR1/mpr1 [Ts932]	Rinaldi et al., 1994
R117/a12	MATa, his1, ade2, leu2, ura3, mpr1 [rho+]	Rinaldi et al., 1994
W303/5	MATa/MATα, his3-11/his3-11, ade2-1/ade2-1, leu2-3,112/leu2-3,112, ura3-1/ura3-1, trp1-Δ2/trp1-Δ2, can1-100, MPR1/mpr1∷URA3 [rho+]	This study
FY1679	MATa/MATα, his3-Δ200/+, leu2-Δ1/+, ura3-52/ura3-52, trp1-Δ63/+, [rho+]	Winston et al., 1995
FY1679/1	MATa/MATα, his3-Δ200/+, leu2-Δ1/+, ura3-52/ura3-52, trp1-Δ63/+, MPR1/mpr1∷URA3, [rho+]	This study
W303-pI	MATα, his3-11, ade2-1, leu2-3,112, ura3-1, trp1-Δ2, mpr1∷pYI-mpr1, can1-100 [rho+]	This study
W303-MPR1	W303-pI with the integrative plasmid pYI-mpr1 excised	This study
W303-mpr1	W303-pI with the integrative plasmid pYI-MPR1 excised	This study
W303-mpr1 [rho°]	MATα, his3-11, ade2-1, leu2-3,112, ura3-1, trp1-Δ2, mpr1, can1-100 [rho°]	This study
W303-MPR1 [rho°]	MATα, his3-11, ade2-1, leu2-3,112, ura3-1, trp1-Δ2, MPR1, can1-100 [rho°]	This study

Plasmid	Insert	Vector	Reference
pYC		YCp50	Rose et al., 1987
pYC31	MPR1; 3100 bp	YCp50
YCpMPR1	MPR1, 2400 bp	YCp50
YEpMPR1	MPR1, 2400 bp	YEplac181	Gietz and Sugino, 1988
KSpMPR1	MPR1, 2400 bp	pBluescript KS+ (Stratagene)
pKSmpr1∷URA3	mpr1∷URA3, 4000 bp	pBluescript KS+
pCRII-mpr1	mpr1, 1625 bp	pCRII (Invitrogen)
pYI-mpr1	mpr1, 1625 bp	YIplac211	Gietz and Sugino, 1988
TU65	GFP (green fluorescent protein), 800 bp	pBluescript KS+ (Stratagene)	Chalfie et al., 1994
pRSETB8	GFP (green fluorescent protein), 800 bp	pRSETB (Invitrogen)
p100GFP	GFP under UASga11-10/CYC1 promoter	pEMBLYex4	Baldari et al., 1987
pKS-EcoRI	pKS without EcoRI restriction site	pBluescript KS+
KSpGFP	GFP, 800 bp	pBluescript KS+
pCRII-MPR1	MPR1, 1452 bp	pCRII
KSpMPR1-GFP	MPR1-GFP, 2250 bp	pBluescript KS+
YEpMPR1-GFP	MPR1-GFP, 2250 bp	YEplac181	Gietz and Sugino, 1988
YCpMPR1-GFP	MPR1-GFP, 2250 bp	YCplac111	Gietz and Sugino, 1988
pSK15	H. sapiens POH1 cDNA, 1500 bp	pBluescript SK+
pYES-POH1	H. sapiens POH1 cDNA, 1500 bp	pYES2 (Invitrogen)