N content of wild-type (WT), atptr1, atptr5, atptr1 atptr5, and 35S:AtPTR5 seedlings on medium containing different N sources. Seeds were germinated and cultivated for 2 weeks on different media as described in the Figure 8 and 9 legends. Data were obtained from three independent experiments. Values are the means of N ±sd (% of dry weight [DW]). Different letters indicate statistically significant differences compared to wild type (Student's t test). a, P < 0.005; b, P < 0.05.