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Localization of AtPTR5/GFP fusion proteins at the plasma membrane of tobacco protoplasts. Confocal laser-scanning microscope images (A, C, E) and corresponding bright-field images (B, D, F) of tobacco protoplasts transiently expressing fusion proteins of GFP with AtPTR5 or GFP. A and B, AtPTR5-GFP. C and D, GFP-AtPTR5. E and F, GFP. Merged images show GFP fluorescence (green) and chlorophyll fluorescence (red). Diameter of protoplasts is approximately 40 μm.
