Figure 4.

Expression analysis of AtPTR5 in Arabidopsis. A, Expression was analyzed by using semiquantitative RT-PCR with the actin mRNA (Act, At3g18780) as a reference. RNA from source leaves, stems, roots, flowers, and siliques of soil-grown plants was analyzed. Similar results were obtained using RNAs from three independent experiments as template. B to H, Expression of the uidA gene under control of the AtPTR5 promoter. AtPTR5-GUS expression in 6-week-old plants (B), 2-week-old seedlings (C), flowers before pollination (stage 12; D), and after pollination (stage 14; E), in vitro-germinated pollen (F), and developing seeds (G and H). No staining could be detected in mature seeds and during seed germination and seedling establishment. Bars = 2,000 μm in C, 25 μm in F, 200 μm in H, and 500 μm in D, E, and G.