Figure 2.

FR- and ¹O₂-mediated peroxidation of 18:3 in Arabidopsis. A, ROS-mediated LPO, expressed as HOTE levels, and HOTE signature (for details, see Fig. 1) of wild-type (Col-0) cell suspensions and roots under control conditions (Con.) and after treatment with tert-BOOH or H₂O₂ for 4 h. B to D, ROS-mediated LPO and HOTE signature (for details, see Fig. 1) in wild-type (Col-0) and vte1 npq1 leaves of plants grown under low light (LL = 100 μmol m⁻² s⁻¹). B and C, Plants were kept in the dark and infiltrated with a mixture of 100 mm BOOH/5 mm Fe²⁺ for 4 h. D, Plants were stressed at 7°C, high light (HL = 1,300 μmol m⁻² s⁻¹), and different CO₂ (n CO₂ = 380 ppm, h CO₂ = 2,000 ppm) conditions for 26 h. Results are expressed as mean ± sd (n = 3). E and F, Images of wild-type (E) and vte1 npq1 (F) plants stressed with high light (1,300 μmol m⁻² s⁻¹; 7°C) for 26 h at 2,000 ppm CO₂ showing leaf damage to the vte1 npq1 plant.