The effect of labeled precursor on predicted relative flux errors (flux error/flux). Simulations used the network illustrated in Supplemental Figure S1. A relative error of less than one (indicated by the dashed line) indicates that the predicted flux error is less than the magnitude of the flux itself. Abbreviations: CO2 (CO2 output), cALD (cytosolic aldolase), pALD (plastidic aldolase), PK (pyruvate kinase), G6PDH (Glc-6-P dehydrogenase), PDH (pyruvate dehydrogenase), SDH (succinate dehydrogenase), MDH (malate dehydrogenase), PEPC (PEP carboxylase), ME (mitochondrial NAD-ME).