Distribution of flux solutions during replicate fitting for the standard (○) and elevated (•) O2 datasets. A, Net fluxes. B, Exchange [0,1] fluxes. The best (lowest sum of squared residuals) 25 solutions for each dataset are shown, with each point representing a single solution. Exchange fluxes (Xch [0,1]) are expressed on a normalized scale following hyperbolic transformation, defined by Xch [0,1] = JXch/(1 + JXch), where JXch is the true exchange flux. Net (J), exchange, forward (Jf), and reverse (Jr) fluxes are related by J = Jf − Jr and JXch = min (Jf, Jr) (Wiechert et al., 2001).