Figure 1.

Functional expression of AtCHX13 in yeast mutant LMM04. A, LMM04 cells expressing vector, AtCHX13, or KAT1 were grown overnight in selection medium (SC-Ura) supplemented with 100 mm KCl. Cultures were brought to a uniform cell density and 5-fold serial dilutions were spotted on SC-Ura medium at various pHs and varying concentrations of KCl. Photos were taken after 4 d of growth at 30°C. B, Cation content of LMM04 yeast expressing empty vector, AtCHX13, or KAT1. All yeast strains were grown overnight in SC medium plus 100 mm KCl and then harvested and washed with K⁺-free medium. Two hundred microliters of each cell culture was then transferred to 5 mL of low K⁺ medium (supplemented with 5 mm KCl). After overnight growth, yeast cultures were collected onto membranes and washed three times with water for inductively coupled plasma analysis. Data are presented as means ± sd (n = 5). One-way ANOVA with Tukey's multiple comparison procedures was used for pairwise comparisons.