Table I.
Content of cytokinin and ABA in basal, middle, and apical segments (2 cm) of detached barley leaves as affected by their incubation for 24 h in the light or in darkness
Cytokinin (trans-zeatin and zeatin riboside) and ABA were determined by ELISA using polyclonal antibodies against cytokinins and ABA.
| Treatment | Phytohormone | Content of Phytohormones in Leaf Segment
|
||
|---|---|---|---|---|
| Apical | Middle | Basal | ||
| ng g−1 fresh weight | ||||
| Leaf immediately after detachment | Cytokinin | 37 ± 2 | 30 ± 2 | 15 ± 1 |
| ABA | 55 ± 2 | 75 ± 2 | 64 ± 2 | |
| 24 h of incubation in darkness | Cytokinin | 50 ± 3 | 48 ± 3 | 26 ± 2 |
| ABA | 94 ± 3 | 194 ± 6 | 121 ± 5 | |
| 24 h of incubation in the light | Cytokinin | 76 ± 5 | 62 ± 4 | 32 ± 2 |
| ABA | 375 ± 11 | 498 ± 15 | 762 ± 29 | |