Figure 1.

Impact of reduced mitochondrial SAT levels on the growth rate of Arabidopsis. A, Wild-type Arabidopsis plants were transformed with pBinAR-amiSAT3 and selected on kanamycin-containing medium. B, After 2 weeks, surviving transgenic plants were transferred to soil for an additional 6 weeks. C, The abundance of mitochondrial SAT3 was measured by immunological detection from soluble Arabidopsis protein extracts (15 μg) using a SAT3-specific antibody (Wirtz and Hell, 2007). The large subunit of ribulose-1,5-bisphosphate carboxylase (LSU) served as a loading control after staining with Coomassie Brilliant Blue. D, At the same time of harvesting the sample for protein extraction, the rosette diameters of the transgenic plants were measured three times for each individual and plotted against the SAT abundance of the corresponding plant. The data were fitted using a linear equation. Error bars indicate sd. [See online article for color version of this figure.]