Figure 7.

Responses of wild-type (wt) and GO lines to high light treatment. A and B, Phenotypes of the wild-type and GO plants (A) and quantification of the anthocyanin levels (expressed as corrected A₅₃₅ per milligram fresh weight) at different times during growth at 600 μmol quanta m⁻² s⁻¹ and ambient CO₂ concentration (380 μL L⁻¹; B). The values are means ± se of three measurements of at least six plants each. FW, Fresh weight. C, Semiquantitative RT-PCR analysis of PAL1, CHS, and DFR transcript levels in leaves of wild-type, GO, and GO-MS plants after 6 h of high light exposure. PCR products of 251 bp (PAL1), 448 bp (CHS), and 450 bp (DFR) were amplified using 33 cycles. As a loading control, a 521-bp ACTIN2 cDNA fragment was amplified using 29 cycles.