Figure 5.
Histogram of Pf of leaf protoplasts (A) and statistical analysis (B) from Arabidopsis wild-type (Col) and transgenic (35S∷Rh-PIP2;1) plants. Leaves of 20- to 22-d-old plants were harvested, and three different preparations of protoplasts were used. For each line, the Pf of 75 protoplasts was measured, and the initial diameter of protoplasts was about 60 μm. Error bars represent se (n = 75). The double asterisk indicates a significant difference in Pf between wild-type (Col) and transgenic (35S∷Rh-PIP2;1) plants (n = 75; t test; P < 0.01).