Figure 7.
BiFC evidence for GGH heterodimer formation in planta. A, Representative fluorescence (i) and bright-field (ii) images of Arabidopsis protoplasts transiently expressing YFP, its N-terminal (YFPN) and C-terminal (YFPC) fragments, or YFPN and YFPC as fusions to LeGGH1, LeGGH2, and/or the signal peptide of LeGGH1 (sp1), as indicated on the right of the images. B, Percentage of the transformed protoplasts above exhibiting BiFC signals from a minimum of three independent experiments. BiFC intensity was visually scored as low, medium, or high (inset) from a minimum of 100 protoplasts viewed for each transformation. Note the lack of high intensity BiFC signals from protoplasts transformed with YFPN/YFPC or GGH1-YFPN/sp1-YFPC combinations.