Figure 5.

Application of the HO-1 potent inhibitor ZnPPIX to auxin-depleted cucumber explants inhibits the IAA and hematin response. Auxin-depleted cucumber explants were incubated with hematin (10 μm), IAA (10 μm), ZnPPIX (200 μm), 10% or 100% saturation of CO aqueous solutions alone, or the combination solutions, then root number per explant was recorded after 5 d of treatment (A). HO activity and CO content were assayed after the indicated times (B, 3 h; C, 24 h). Bars denoted by the same letter did not differ significantly at P < 0.05 according to Duncan's multiple test. FW, Fresh weight.