Figure 6.

IAA dose-dependently induces HO activity (A) and HO-1 protein expression (B) in auxin-depleted cucumber explants. Explants were incubated with IAA at the indicated concentrations for 6 h. Coomassie Brilliant Blue-stained gels (C) are present to show that equal amounts of proteins were loaded. Bars with asterisks were significantly different in comparison with water treatment (0 μm IAA) at P < 0.05 and P < 0.01 according to t test (A). Three independent experiments were performed, and the immunoblotting results show similar trends of protein expression (B).