Figure 7.

In vitro NO generation in crude protein extracts of leaves shows Ca²⁺/CaM dependence. A, NO generation in the absence (white bar) and presence (gray bar) of the NR inhibitor (1 mm) NaN₃. B, NO generation in the absence (white bar) and presence (gray bar) of the NOS inhibitor (20 μm) DPI. C, Effect of the Ca²⁺ chelator EGTA on NO generation. D, All assays included 1 mm NaN₃. In the presence of this NR inhibitor, NO generation was monitored in the presence and absence of 1 mm Ca²⁺ and 1 μm CaM. E, In the presence (left) or absence (right) of 1 μm CaM, NO generation was monitored in the absence (white bars) or presence (gray bars) of 200 μm W7. All assays in A to C included 1 mm Ca²⁺ and 1 μm CaM. Assays in E included 1 mm Ca²⁺. All results are presented as mean arbitrary fluorescence units ± se (n =3). Each experiment was repeated two to four times.