FIG. 3.
Specific cytolytic responses induced in mice by transfectant influenza A viruses expressing the β-gal epltope TPHPARIGL To evaluate the function of transfectant influenza A viruses in the priming of β-gal-specific cytotoxic responses in vivo, two mica/group were injected intravenously with 106 PFU of the influenza A virus shown on the abscissa. Three weeks later, splenocytes from immunized mice were cultured in the presence of the Ld-restricted β-gal876–884 peptide for 6 days, then tested in a microcytotoxicity assay against CT26.WT, CT26.CL25, or CT26.WT cells loaded with the β-gal876–884 peptide at the indicated E:T ratios. Experiment was repeated with similar results.