Skip to main content
. Author manuscript; available in PMC: 2008 Dec 11.
Published in final edited form as: Biochemistry. 2007 Nov 14;46(49):14188–14197. doi: 10.1021/bi701363s

Table 1.

Kinetic isotope effect of the wild-type and Y94F TS on proton abstraction at 25 °C.

KIE Wild Type Y94F
T(V/K)Ha 1.52 ± 0.03 1.44 ± 0.03
T(V/K)Da 1.17 ± 0.01 1.15 ± 0.01
H/T KIEint 3.2 ± 0.2 3.2 ± 0.3
D/T KIEint 1.41 ± 0.03 1.42 ± 0.04
H/D KIEint 2.2 ± 0.1 2.3 ± 0.2
a

At 5 µM CH2H4folate.