Table 2.

Rates and isotope effect on Arrhenius parameters of the wild-type and Y94F TS on hydride transfer^a.

	Wild typeb	Y94F		SC AL/ATc
	5 – 40 °C	5 – 20 °C	20 – 40 °C
AH/AT	6.9 ±1.0	0.0002 ±0.0001	4.5 ±0.5	0.5 –1.6
AD/AT	1.8 ±0.1	0.07 ±0.02	1.6 ±0.1	0.9 –1.2
ΔEaH-T	0.02 ±0.09	6.0 ±0.5	0.03 ±0.40
ΔEaD-T	0.01 ±0.02	1.8 ±0.2	0.06 ±0.12
Ea	4.0 ±0.3	6.2 ±0.3	3.1 ±0.1
ΔH‡	3.4 ±0.08	5.6 ±0.3	2.5 ±0.1
TΔS‡25°C	−13.7 ±0.7	−14.5 ±0.5	−17.6 ±0.7

^aAll energy units are kcal/mol.

^bFrom ref. (10).

^cSemi-classical limits of isotope effects on preexponential factor of Arrhenius equation.(35,45,46,53).