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. Author manuscript; available in PMC: 2008 Dec 11.
Published in final edited form as: Biochemistry. 2007 Nov 14;46(49):14188–14197. doi: 10.1021/bi701363s

Table 3.

Observed kcat and Tkcat used to assess the values of khydride at 5, 20, and 40 °C using Eq. 10a.

wild typeb Y94F

Temp. [°C] kcat [s−1] kcat [s−1] Dkcat khydride [s−1]
40 1.6 0.0067 2.24 0.0109
20 1.1 0.0055 2.36 0.0080
5 0.73 0.0023 4.20 0.0026
a

All relative errors are under 2 % of the measured values and under 6 % of the calculated khydride.

b

From ref. (10).