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. Author manuscript; available in PMC: 2008 Oct 1.
Published in final edited form as: Biochemistry. 2005 Dec 6;44(48):15774–15786. doi: 10.1021/bi0515974

Figure 4. Exonuclease and NDP kinase activity of purified human NDK1 mutants.

Figure 4

A. Ni-NTA column-purified human wild type and mutant NDK1 proteins on an SDS-PAGE gel. B. Exonuclease activity of purified human wild type and mutant NDK1 proteins was tested with a single-stranded 30-mer oligonucleotide and equal amounts (100 ng) of purified proteins. C. Enzymatic assay for nucleoside diphosphate kinase activity. D54A, E5Q and E5A mutants retained relatively high kinase activity.