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. Author manuscript; available in PMC: 2008 Oct 1.
Published in final edited form as: Biochemistry. 2005 Dec 6;44(48):15774–15786. doi: 10.1021/bi0515974

Figure 5. Competition assay between human NDK1 wild type and mutants with a single stranded oligonulceotide substrate.

Figure 5

A. The competition effect of E129A and E129Q mutants on wild type NDK1 exonuclease activity is shown. Various amounts of E129A and E129Q proteins were preincubated with the single-stranded oligonucleotide substrate followed by incubation with 0.5 μg of wild type human NDK1. B. E5A and E5Q mutants were used for the competition assay. C. Increasing amounts of wild type NDK1 were used with the same substrate. D. Quantified data of the competition effects by human NDK1 mutants. The cleaved substrates were quantified by phophoimager analysis and converted to percentage values relative to wild type NDK1.