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. Author manuscript; available in PMC: 2008 Oct 1.
Published in final edited form as: Biochemistry. 2005 Dec 6;44(48):15774–15786. doi: 10.1021/bi0515974

Figure 9. Western blot analysis of human NDK1 in HeLa and ARPE-19 cells.

Figure 9

A. Cytoplasmic and nuclear extracts from DNA damaging agent-treated HeLa cells were separated on SDS-PAGE. Endogenous human NDK1 in both cytoplasmic (C) and nuclear extracts (N) was detected with anti-human NDK1 antibody. HDAC2 was used as a control for the nuclear protein fraction and as a loading standard. β-tubulin was used as a cytoplasmic specific and loading control. Cells were lysed 20 min after DNA damage induction. The “increased ratio” refers to the relative increase in the percentage of nuclear localized protein in damaging agent-treated cells relative to untreated cells, corrected for the values of the loading controls. B. Localization analysis of human NDK1 in untreated and DNA damaging agents-treated ARPE-19 cells.