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Expression of Twist1 and phosphoregulatory mutants in mouse limbs. Monomer-Prx constructs were used to generate E17.5 F0 embryos. Embryos were stained with Alcian blue and Aiizarine S as described (17) from 1 wild type and 3-transgenic positive littermates. Limbs were dissected away for detailed analysis. Filled arrowhead, clavicle; filled circle, deltoid tuberosity; gray arrowhead, ulna; dotted arrowhead, scapula; open arrowhead, patella; cross, fibula; v, iliac bone.
