. Author manuscript; available in PMC: 2008 Oct 1.

Published in final edited form as: Biochemistry. 2006 Jun 20;45(24):7415–7428. doi: 10.1021/bi060152s

Table 4.

Kinetic constants for L. casei Tyr-261 mutants and E. coli Y209W

Mutant	k_cat, s^-1	K_m^dUMP, μM	K_m^CH2H4folate, μM	K_m^dUMP/K_m^CH2H4folate	K_d^dUMP
WT LcTS	3.8+/- 0.02	6+/- 2	17+/- 3	0.35	0.39+/- 0.05
Y261F	1.7+/- 0.04	6.6+/- 0.6	69+/- 13	0.08	0.44+/- 0.65
Y261M	0.26+/- 0.02	7+/- 2	68.7	0.10
Y261A	0.026+/- .001	34+/- 6	221+/- 5	0.15
Y261W	0.022+/- .001	78+/- 11	119.9	0.65
WT EcTS^a	8.8	4.1+/- 0.4	13.6+/- 0.2	0.29
Y209W	0.05+/- 0.01	118+/- 40	172 +/- 26	0.68

Data from Reyes et al. (51).