Figure 2.

v-Src–transformed myoblasts expressing Rho family GTPases display distinct phenotypes. Immunofluorescence micrographs of QMb-LA29 kept in GM at 35°C after transfection with Rac1L61 (A and F), Cdc42L61 (B and G), RhoAV14 (C and H), Rac1N17 (D and I), and β-galactosidase (E and J) labeled with BODIPY phallacidin (A–E) and antibody to vinculin (F–J). Cells expressing the transfected constructs were identified by double labeling with antibody to the myc epitope or to β-galactosidase (arrows). Bar, 10 μm.