Table 2.

Primers used in this study

Primer	Sequence (5′-3′)	Accession number and corresponding sequence (nt)
del-AW-F1-BH1	CGGCATCCTGGTGAGTTTATCTCGATTGC	DQ150110 (180→200)
del-AW-R1	GTCTAAATCAATTTTATTAAAGTTCATCAAGCTCTTGTTCACCTCAATGG	NC002013 (1286→1260 (minus strand) ), DQ150110 (1044→1022 (minus strand) )
del-AW-F-CAT	CCATTGAGGTGAACAAGAGCTTGATGAACTTTAATAAAATTGATTTAGAC	DQ150110 (1022→1044), NC002013 (1260→1286)
del-AW-R-CAT	CCTTTGCTCTATGTATTTATTTCTTTCATTATAAAAGCCAGTCATTAGGCC	DQ150110 (3629→3604 (minus strand) ), NC002013 (1911→1888 (minus strand) )
del-AW-F2	GGCCTAATGACTGGCTTTTATAATGAAAGAAATAAATACATAGAGCAAAGG	NC002013 (1888→1911), DQ150110 (3604→3629 )
del-AW-R2-ER1	CGGAATTCTAGTCGTATGATCGTCACCAG	DQ150110 (4481→4461 (minus strand) )
OF4cheA3	TGAGAGCCTAGAAGCAGCAT	DQ150110 (1419→1438)
OF4cheA15	TGACGATGCCGCCTAATGATTC	DQ150110 (2674→2653 (minus strand) )
OF4cheA24	CGCGATGTGTATCAAGAGTTCTC	DQ150110 (1→23 )
OF4cheA25	CCGGTGAGGGTGACCATATTCATT	DQ150110 (4858→4835 (minus strand) )
NC-F1-SC2	TTTCCGCGGGCCCTTAGGAGGAATTCTGT	AY376071 (8→30)
NC-R1-1	GAGCTCCTCGCCCTTGCTCACCATCCCTTTTTTTGTTTGCTGTTTATG	a) (312→289 (minus strand) ), AY376071 (1729→1706 (minus strand) )
NC-F-CFP1	CTGAAACAGCAAACAAAAAAAGGGATGGTGAGCAAGGGCGAGGAGCTG	AY376071 (1706→1729), a) (289→312)
NC-R-CFP1	CTGTCTTTAGTCAGCTCCTTTTTGTTACTTGTACAGCTCGTCCATGCC	AY376071 (1756→1733 (minus strand) ), a) (1008→985 (minus strand) )
NC-F2-1	GGCATGGACGAGCTGTACAAGTAACAAAAAGGAGAAGACTAAAGACAG	a) (985→1008), AY376071 (1733→1756)
NC-R2-BH1	GAGGATTCCCGGAGTGGAAGCTGACTTATA	AY376071 (2510→2531 (minus strand) )
CN-R1-1	CAGCTCCTCGCCCTTGCTCACCATTTACATAATAACTCCTTCTTGCAT	a) (312 → 289 (minus strand) ), AY376071 (876→854 (minus strand) )
CN-F-CFP-1	ATGCAAGAAGGAGTTATTATGTAAATGGTGAGCAAGGGCGAGGAGCTG	AY376071 (854→876) a) (289→312)

Extra nucleotides that were added to introduce restriction sites are underlined.

^a)Sequence data from http://orders.clontech.com/clontech/techinfo/vectors_dis/pECFP.shtml