Table 3.
Quantification of the co-localization of NaVBP and McpX
| Strain | Subcelluar localization of NaVBP (%) | ||||||||
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| A | B | C | D | E | A+B+C+D | A+B+C+D+E | Total fluorescent cells (%) | Total cells | |
| One pole | Two poles | One pole and side(s) | Two poles and side(s) | Side(s) | Polar localization | Total | |||
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| 811M | 28 | 16 | 20 | 12 | 9.8 | 82 | 92 | 100 | 153 |
| (Wild type) | (86)a | (90)b | |||||||
| SC34 | 1.2 | 0.63 | 0 | 0 | 2.5 | 1.9 | 4.4 | 100 | 158 |
| (ΔncbA) | (50)a | (67)b | |||||||
| SC34-R | 52 | 14 | 7.1 | 2.1 | 13 | 76 | 89 | 100 | 141 |
| (ΔncbA, ncbA restored) | (89)a | (89)b | |||||||
| 811M-cheAW | 25 | 2.0 | 6.7 | 0 | 16 | 33 | 49 | 100 | 150 |
| (cheAW disrupted) | (49)a | (50)b | |||||||
| Strain | Subcelluar localization of McpX (%) | ||||||||
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| A | B | C | D | E | A+B+C+D | A+B+C+D+E | Total fluorescent cells (%) | Total cells | |
| One pole | Two poles | One pole and side(s) | Two poles and side(s) | Side(s) | Polar localization | Total | |||
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| 811M | 38 | 17 | 12 | 14 | 15 | 78 | 93 | 100 | 153 |
| (Wild type) | (91)c | (94)d | |||||||
| SC34 | 10 | 3.2 | 1.9 | 0 | 8.9 | 15 | 24 | 100 | 158 |
| (ΔncbA) | (6.3)c | (4.2)d | |||||||
| SC34-R | 49 | 13 | 5.0 | 2.1 | 13 | 71 | 84 | 100 | 141 |
| (ΔncbA, ncbA restored) | (96)c | (96)d | |||||||
| 811M-cheAW | 23 | 4.0 | 5.3 | 0 | 17 | 33 | 49 | 100 | 150 |
| (cheAW disrupted) | (54)c | (51)d | |||||||
The number in parentheses is the % of the cells with NaVBP localized at one pole that also had McpX localized at one pole.
The number in parentheses is the % of the cells with NaVBP localized to at least one pole (A+B+C+D) that also had McpX co-localized to at least one pole.
The number in parentheses is the % of the cells with McpX localized at one pole that also had NaVBP localized at one pole; this number correlated well with overlay measurements.
The number in parentheses is the % of the cells with McpX localized at at least one pole (A+B+C+D) that also had NaVBP co-localized at at least one pole.