Table 5.
Swarming assay and tumbling bias of B. pseudofirmus OF4 strains
| Strain | Colony diameter (mm)a | Tumblingb |
|---|---|---|
| 811M (wild-type) | 17.5 ± 2.0 | ± |
| SC34 (ΔncbA) | 11.8 ± 1.0 | ++ |
| SC34 / pSC-CFP | 15.8 ± 1.9 | ± |
| SC34 / pCFP | 12.0 ± 0.5 | ++ |
| SC34-R (ΔncbA, ncbA restored) | 17.0 ± 1.8 | + |
Measured after 10 hr on MYE medium, pH 10, solidified with 0.3% agar. Mean values of three independent experiments with standard deviations of the mean.
Qualitative assessment of tumbling bias in liquid MYE medium (pH 10) by the hanging drop method using a dark field microscope.