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. Author manuscript; available in PMC: 2008 Oct 1.
Published in final edited form as: Science. 2007 Feb 22;315(5818):1576–1579. doi: 10.1126/science.1137999

Fig. 3.

Fig. 3

Soft-agar assay for anchorage-independent growth. (A) Hmga2 constructs used for stable transfection, depicted as in Fig. 2A. (B) Colony formation. For cells stably transfected with the indicated vector, the percentage that yielded colonies after 28 days is plotted (horizontal line, median; box, 25th through 75th percentile; error bars, range; n = 12 from four independent experiments, each in triplicate). All but Hmga2-wt yielded a significantly higher number of colonies than did the empty vector (Mann-Whitney test for each, P < 0.05). When compared with Hmga2-wt, a significantly higher number of colonies was observed for Hmga2-tr (P = 0.003). Hmga2-m7 showed significantly more colonies than any of the other constructs tested (P < 0.05 for each; *, P = 0.041; **, P = 0.002; ***, P = 10−6). No significant difference was observed between Hmga2-wt and the construct with the truncated ORF (P = 0.61).