Table 1. Primer sequences of the genes studied in the RT–PCR assay.
| Primer | Gene | Forward primer (5’-3’) | Reverse primer (5’-3’) |
|---|---|---|---|
| Endogenous controls |
RPS-11 |
AAGCAGCCGACCATCTTTCA |
CGGGAGCTTCTCCTTGCC |
| |
HRPT |
CGAGATGTGATGAAGGAGATGG |
GCAGGTCAGCAAAGAATTTATAGC |
| |
β-ACTIN |
CGGGACCTGACTGACTACCTC |
CTCCTTAATGTCACGCACGATTT |
| Genes under study |
VEGF-A |
GCCCTTGCCTTGCTGCTCTACC |
GTGATGATTCTGCCTCCTCCTTC |
| |
PEDF |
AGCATTCTCCTTCTCGGTGTGG |
CCTCACGGTCCTCTCTTCATCC |
| TSP-1 | AGGTCTTCAGCGTGGTGTCC | ACAAACAGGGTGATGCTCTTCC |
Primer sequences of the control and studied genes that were used in real-time quantitative RT-PCR assay to determine level of expression in UM cell lines. Abbreviations: β-ACTIN is beta-actin; HPRT is hypoxantine phosphoribosyltransferase; RPS-11 is ribosomal protein S11.