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. Author manuscript; available in PMC: 2009 Aug 15.
Published in final edited form as: J Immunol. 2008 Aug 15;181(4):2855–2868. doi: 10.4049/jimmunol.181.4.2855

Figure 3. CD27, 4-1BB, CD40L, OX40 or ICOS costimulation cannot substitute for CD28 costimulation to promote Treg expansion.

Figure 3

A.2×105 enriched Tregs were stimulated with a cell-based aAPC that delivered the indicated costimulatory signal and cultured the presence of rapamycin. The population doubling rates were measured by cell counting. Each data point represents the average of three independent experiments (error bars represent standard deviation). B and C Analysis of the cell populations expanded in A. after 14 days culture for Foxp3 (B) and CD62-L and CD27 (C) expression.