Figure 1.

Organization of azurophil granules and vimentin in fMLP-stimulated neutrophils. Dual-label immunofluorescence localization of MPO (left panel) and vimentin (right panel) is shown in control neutrophils (A and B) and cells stimulated with 0.1 μM fMLP for 30 s (C, and D), 1 min (E and F), 2.5 min (G and H), and 5 min (I and J). fMLP induced a transient change in vimentin organization. Intermediate filaments organized at 30 s and were localized within areas of the cell devoid of granules at 1 min and 2.5 min. By 5 min the cells were smaller and vimentin filaments were distributed throughout the cytoplasm with the granules. Magnification, 2500×.