Figure 7.

The effects of LY83583 and 8-Br-cGMP on neutrophil granule secretion. Neutrophils were stimulated with fMLP for various times in the absence (▪) or presence of 1 μM 8-Br-cGMP (▵) or 100 μM LY83583 (♦). LY83583 inhibited secretion of LF (A) and MPO (B). Granule secretion was accelerated and augmented by 8-Br-cGMP. Analysis of variance utilizing Student’s paired t test showed that 8-Br-cGMP significantly enhanced LF and MPO secretion (p < 0.01), and that LY83583 significantly inhibited secretion at all time points (p < 0.001). Secretion was significantly restored by the addition of 1 μM 8-Br-cGMP to LY83583-treated cells (E) at 5 min for LF and MPO (p < 0.001). Data are from one of three experiments performed on a single blood donor. All three experiments demonstrated the same trends.