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. 2008 Sep 17;8:72. doi: 10.1186/1472-6750-8-72

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Copyright © 2008 Dib and Nidetzky; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Interfacial inactivation of free and immobilized TvDAO preparations. Loss of activity induced by bubble aeration in a miniaturized stirred reactor is shown for the different enzyme preparations and compared to a non-aerated control of soluble TvDAO (full down triangles). Experiments were performed at 30°C with stirring at 300 rpm and an aeration rate of 15 L/h; protein concentration was 1 μM in Tris-buffer. Symbols: full triangles, soluble enzyme; open triangles, soluble enzyme in presence of 1% Pluronic F-68; open squares, TvDAO immobilized on Strep-Tactin MacroPrep beads; full circles, TvDAO on Sepabeads EC-EP.