Skip to main content
. Author manuscript; available in PMC: 2009 Jan 1.
Published in final edited form as: J Neuroimmunol. 2007 Dec 11;193(1-2):68–76. doi: 10.1016/j.jneuroim.2007.10.016

Fig. 5. Uveitogenic activity of IRBP168-177-activated IRBP161-180-specific T cells.

Fig. 5

Nylon wool-enriched splenic T cells, prepared from IRBP161-180-immunized B10RIII mice at day 13 p.i., were subjected to in vitro stimulation with 20 μg/ml of IRBP161-180 or IRBP168-177 and APCs (the control group was exposed to APC alone in the absence of peptide), then 5 ×106 T cell blasts, separated by Ficoll gradient centrifugation, were transferred to each groups (n=4) of recipient mouse. The clinical score was monitored by fundoscopy and the eyes of the recipient mice subjected to pathological examination 15 days later. Eye histology showed that IRBP168-177-stimulated T cells induced modest, but significant, EAU upon adoptive transfer to naïve B10RIII mice.