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. 2008 Oct 6;183(1):11–17. doi: 10.1083/jcb.200807052

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© 2008 Jang et al.

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Figure 1. — A genetic modifier screen for Drosophila LKB1 identifies a novel LKB1-interacting gene, Sln. (A) A schematic illustration of a genetic modifier screen for LKB1. (B–D) Scanning electron microscopy images of adult eyes (B) and images of adult wings (C and D) from the indicated genotypes. The penetrances of the eye and wing phenotypes were 100% (n = 100). (E) Genomic locus of the Sln gene. (F) RT-PCR analysis of Sln transcript in adult flies expressing hs-gal4 alone or hs-gal4–driven Sln^EP1. rp49 was used as a loading control. (G) Developmental Northern blot analysis of Sln. E, embryo; L, larvae; P, pupa; F, adult female; M, adult male. 28S rRNA was used as a loading control. (H) Whole-mount in situ hybridization analyses of Sln in wild-type larval eye (left) or wing (right) discs with an antisense (top) or a sense probe (bottom).