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. 2008 Oct 6;183(1):101–116. doi: 10.1083/jcb.200801099

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© 2008 Degtyarev et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 1. — Inducible KD of Akt isoforms and their effect on xenograft tumor growth. (A) Immunoblot analysis of Akt isoforms and various downstream proteins in stable PC3 clones expressing the inducible shRNA constructs. Each clone was induced to express the respective shRNAs with 1 μg/ml Dox grown under 10% FBS for 7 d. Double arrowheads indicate slight differences in the mobility of the three Akt isoforms detected by total and phospho-Akt antibodies and the mobility shift of IRS1. (B) Effect of Akt KD on xenograft tumor growth. Representative experiments showing the growth of PC3 xenograft tumors containing the various shRNAs treated with vehicle control (−Dox, closed circles) or Dox (+Dox, open circles; see Table S2, available at http://www.jcb.org/cgi/content/full/jcb.200801099/DC1). Error bars represent SEM. *, P < 0.05; **, P < 0.005.