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. 2008 Oct 6;183(1):101–116. doi: 10.1083/jcb.200801099

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© 2008 Degtyarev et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 2. — Akt KD resulted in cell cycle delay without substantial apoptosis. (A) Histological analysis of PC3-shAkt123 tumors treated with Dox or vehicle control for 5, 15, or 21 d as indicated. Tumor tissues were analyzed by IHC using antibodies specific for Ki-67 or by the TUNEL assay. Pathologist's scoring of the signal intensity for each sample is indicated in parentheses. Bars, 100 μm. (B and C) Effect of triple-Akt KD on cell cycle progression under serum starvation (ss) compared with cells grown under 10% FBS. Cells containing shRNAs targeting EGFP or all three Akts were pretreated for 2 d with or without Dox in medium containing 10% FBS and changed to 0% (B) or 0.5% (C) FBS. Cell cycle profiles were analyzed at the indicated time points after serum withdrawal. Error bars represent SEM (n = 3). The percentage of change in each cell cycle phase with Dox versus without Dox treatment is also shown.