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. Author manuscript; available in PMC: 2008 Oct 2.
Published in final edited form as: Dev Dyn. 2008 May;237(5):1321–1333. doi: 10.1002/dvdy.21526

Figure 2. Expression of retinoic acid receptors (RARs) and retinoid x receptors (RXRs) in male mouse UGS.

Figure 2

The expression of RARs and RXRs was evaluated at E16.5, during the initiation of prostatic buds from the UGS. Since retinoid activity was most abundant in UGS mesenchyme in Fig. 1, cDNA was synthesized from isolated E16.5 UGS mesenchyme (pooled from n = 5 separate fetuses) and purity of this mesenchymal cDNA preparation was demonstrated by showing that it was deficient in the epithelial-specific gene keratin 14 (Krt14). All six of the Rar and Rxr mRNAs were detected in UGS mesenchyme. Immunoblotting was used to determine which RAR and RXR isoforms were present.